Journal: Oncology Letters

Article Title: lnc-NLC1-C inhibits migration, invasion and autophagy of glioma cells by targeting miR-383 and regulating PRDX-3 expression

doi: 10.3892/ol.2021.12901

Figure Lengend Snippet: Analysis of apoptosis, ROS level and cell cycle in U87MG cells. *P<0.05 and ***P<0.001. n=3. Lnc-NLC1-C, long non-coding RNA narcolepsy candidate region 1 gene C; ov, overexpression; sh, short hairpin RNA; NC, negative control; ROS, reactive oxygen species.

Article Snippet: After 200 µl binding buffer was added to the cells, 10 µl Annexin V-fluorescein isothiocyanate and 10 µl propidium iodide (part of the AnnexinV-FITC/PI Apoptosis Detection kit; cat. no. 556547; BD Biosciences) were added, and the cells were incubated at 4°C in the dark for 30 min and subjected to flow cytometry (FC500 MCL; Beckman Coulter, Inc.).

Techniques: Over Expression, shRNA, Negative Control

Journal: Human Reproduction (Oxford, England)

Article Title: A mouse model of endometriosis mimicking the natural spread of invasive endometrium

doi: 10.1093/humrep/dez253

Figure Lengend Snippet: Endometrial glands formed in ovary are ARID1A-negative. (A) H&E staining in sections of ovarian lesion. (B–D) Immunohistochemistry (IHC) for ARID1A (B), KRT8 (ketatin 8) (C) and phospho-S6 (P-S6) (D) in sections of ovarian lesion. Panels A–D represent near-adjacent sections of extrauterine endometrial lesions, while panels A′–D′ represent portion of slide in panels A–D surrounded by black box. Arrows indicate epithelial gland formation. (E–J) Picrosirius red staining in sections of adjacent uterine tissue (E, F), omental lesions (G) and peritoneal lesions (H–J). Panel J′ represents portion in panel J surrounded by black box. Ab: abdominal wall; Cy: cyst; Fs: fibrotic stroma; Of: ovarian fat; Om: omentum; Oo: oocyte; Ov: ovary: Sr: serosa.

Article Snippet: For picrosirius red staining, the Picrosirius Red Stain Kit (Polysciences, Inc., Warrington, PA, USA, cat# 24901-250) was used according to the manufacturers’ instructions. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Dilution Source Identifier Rabbit monoclonal anti-ARID1A/BAF250A (D2A8U) 1:200 Cell Signaling Technology Cat# 12354; RRID: AB_2637010 Rabbit monoclonal anti-Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) 1:400 Cell Signaling Technology Cat# 4858; RRID: AB_2721245 Rat monoclonal anti-TROMA-I 1:100 Developmental Studies Hybridoma Bank Cat# TROMA-I; RRID: AB_531826 Goat polyclonal anti-ICAM-1/CD54 1:100 R&D Systems Cat# AF796-SP; RRID: AB_2248703 Rat monoclonal anti-FRA-2 (REY146C) 1:200 EMD Millipore Cat# MABS1261 Rabbit monoclonal anti-c-Jun (60A8) 1:300 Cell Signaling Technology Cat# 9165; RRID: AB_2130165 Rabbit monoclonal anti-Ki67 (D3B5) 1:400 Cell Signaling Technology Cat# 12202; RRID:AB_2620142 Open in a separate window RRID, Research Resource Identifier; ARID1A, AT-rich interactive domain-containing protein 1A; ICAM-1, intercellular adhesion molecule 1; Fra2, Fos-related antigen 2; TROMA-1, trophectoderm-specific marker 1.

Techniques: Staining, Immunohistochemistry